When you delete a cohort, you're only deleting the association between the users and this cohort. You are not deleting the users in the system.
Step-by-step Guide
To delete a cohort:
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Go to Site Administration > Users > Cohorts
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Select the "System cohorts" tab
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Click on the delete icon next to the cohort in question
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Confirm the deletion on the next page
Important Notes
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Cohorts created by TeamAlign™ cannot be deleted, as they are automatically generated based on predefined rules and data from integrated systems like your HRIS, HCM, or Active Directory.